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OriGene murine mt2 orf

<t>Mt2</t> expression markedly reduces Neo1 levels in Hep3B cells . A , diagram of Mt2 and fMt2. The MYC (m) and FLAG (f) epitopes were added to the C-terminus of Mt2. B , diagram of fNeo1. The MYC (m) and FLAG (f) epitopes were added to the C-terminus of Neo1. C , coexpression of fMt2 with fNeo1 markedly reduces fNeo1 levels in whole cell extracts (input) and on cell surface by biotinylation. AP, aprotinin. Experiments were repeated three times with consistent results. D , quantification of cell surface fNeo1 bands in C ( panel 4 ). The relative amounts to Na + K + ATPase ( panel-5 ) are presented (n = 3). The data shown are means ± SD. One-way ANOVA was used to analyze the data. ns, no statistical difference. ∗, p < 0.05; ∗∗∗∗, p < 0.0001. NEO1, neogenin.

Murine Mt2 Orf, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/murine+mt2+orf/pmc12887391-307-19-36?v=OriGene
Average 94 stars, based on 3 article reviews

murine mt2 orf - by Bioz Stars, 2026-08

94/100 stars

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1) Product Images from "Matriptase-2-mediated suppression of hepatic hepcidin expression in mice requires hepatocyte neogenin"

Article Title: Matriptase-2-mediated suppression of hepatic hepcidin expression in mice requires hepatocyte neogenin

Journal: The Journal of Biological Chemistry

doi: 10.1016/j.jbc.2026.111142

Mt2 expression markedly reduces Neo1 levels in Hep3B cells . A , diagram of Mt2 and fMt2. The MYC (m) and FLAG (f) epitopes were added to the C-terminus of Mt2. B , diagram of fNeo1. The MYC (m) and FLAG (f) epitopes were added to the C-terminus of Neo1. C , coexpression of fMt2 with fNeo1 markedly reduces fNeo1 levels in whole cell extracts (input) and on cell surface by biotinylation. AP, aprotinin. Experiments were repeated three times with consistent results. D , quantification of cell surface fNeo1 bands in C ( panel 4 ). The relative amounts to Na + K + ATPase ( panel-5 ) are presented (n = 3). The data shown are means ± SD. One-way ANOVA was used to analyze the data. ns, no statistical difference. ∗, p < 0.05; ∗∗∗∗, p < 0.0001. NEO1, neogenin.

Figure Legend Snippet: Mt2 expression markedly reduces Neo1 levels in Hep3B cells . A , diagram of Mt2 and fMt2. The MYC (m) and FLAG (f) epitopes were added to the C-terminus of Mt2. B , diagram of fNeo1. The MYC (m) and FLAG (f) epitopes were added to the C-terminus of Neo1. C , coexpression of fMt2 with fNeo1 markedly reduces fNeo1 levels in whole cell extracts (input) and on cell surface by biotinylation. AP, aprotinin. Experiments were repeated three times with consistent results. D , quantification of cell surface fNeo1 bands in C ( panel 4 ). The relative amounts to Na + K + ATPase ( panel-5 ) are presented (n = 3). The data shown are means ± SD. One-way ANOVA was used to analyze the data. ns, no statistical difference. ∗, p < 0.05; ∗∗∗∗, p < 0.0001. NEO1, neogenin.

Techniques Used: Expressing

Hepcidin is an iron regulatory hormone that is secreted mainly by hepatocytes . A , hepcidin inhibits iron efflux from duodenum, spleen, and the liver into the circulation by blocking the plasma-membrane iron exporter, ferroportin. B , diagram of the key components that are involved in the induction of hepcidin expression in the liver. C , cartoon of NEO1 protein. It contains four immunoglobulin-like (Ig) domain, six fibronectin III domains, a transmembrane domain, and a cytoplasmic domain (CD). D , diagram of MT2 protein. Cyto: cytoplasmic domain. transmembranedomain. SEA: sea urchin sperm protein, enteropeptidase agrin. CUB: complement protein subcomponents C1r/C1s, urchin embryonic growth factor and bone morphogenetic protein one domain. L: low-density lipoprotein receptor class-A domain. Catalytic: serine protease (S/P) catalytic domain. The arrow indicates the predicted autocleavage activation site. E , iron induction of hepcidin expression by increases in Bmp6 gene transcription and TfR2 protein stabilization. F , a proposed model for the function of MT2. NEO1, neogenin.

Figure Legend Snippet: Hepcidin is an iron regulatory hormone that is secreted mainly by hepatocytes . A , hepcidin inhibits iron efflux from duodenum, spleen, and the liver into the circulation by blocking the plasma-membrane iron exporter, ferroportin. B , diagram of the key components that are involved in the induction of hepcidin expression in the liver. C , cartoon of NEO1 protein. It contains four immunoglobulin-like (Ig) domain, six fibronectin III domains, a transmembrane domain, and a cytoplasmic domain (CD). D , diagram of MT2 protein. Cyto: cytoplasmic domain. transmembranedomain. SEA: sea urchin sperm protein, enteropeptidase agrin. CUB: complement protein subcomponents C1r/C1s, urchin embryonic growth factor and bone morphogenetic protein one domain. L: low-density lipoprotein receptor class-A domain. Catalytic: serine protease (S/P) catalytic domain. The arrow indicates the predicted autocleavage activation site. E , iron induction of hepcidin expression by increases in Bmp6 gene transcription and TfR2 protein stabilization. F , a proposed model for the function of MT2. NEO1, neogenin.

Techniques Used: Blocking Assay, Clinical Proteomics, Membrane, Expressing, Activation Assay

Expression of exogenously administered Mt2 in the liver of Tmprss6 −/− mice increases Neo1 levels . A , diagram of Mt2, fMt2, and fMt2 mask . B , experimental design to determine the effects of exogenously administered Mt2 on Neo1 levels. Eight-week-old Tmprss6 −/− littermates on a mixed B6/129 background were intraperitoneally injected with AAV8-fMt2 vectors at ∼8 x 10 11 or ∼4 x 10 12 viral genome-particles per mouse or AAV8-fMt2 mask viral vectors at ∼4 x 10 12 viral genome-particles per mouse. Injection of sterilized PBS vehicle was included as a control. Age and gender-matched WT littermates were included as additional controls. Mice were euthanized for analysis 3 weeks after injection. All mice were fed a standard diet containing 240-ppm iron. Each group consists of at least five mice with similar numbers of male and female. C , qRT-PCR analysis of Tmprss6 mRNA in the liver. Results are expressed as the amount relative to that of β-actin for each sample. The mean values and SD are presented. D , representative images of Western blot analysis for Neo1, the FLAG epitope of fMt2, Tfr2, and β-actin in the liver membrane preparations. E , qRT-PCR analysis of Hamp mRNA in the liver. F , serum iron assay. G ,. quantification of Neo1 bands in D . H , quantification of Tfr2 bands in D . The relative amounts to β-actin are presented. One-way ANOVA was used to analyze the data relative to WT mice. ∗∗, p < 0.01; ∗∗∗, p < 0.001; ∗∗∗∗, p < 0.0001. I , models for Neo1/Hjv induction of hepcidin expression in the absence and presence of Mt2. NEO1, neogenin.

Figure Legend Snippet: Expression of exogenously administered Mt2 in the liver of Tmprss6 −/− mice increases Neo1 levels . A , diagram of Mt2, fMt2, and fMt2 mask . B , experimental design to determine the effects of exogenously administered Mt2 on Neo1 levels. Eight-week-old Tmprss6 −/− littermates on a mixed B6/129 background were intraperitoneally injected with AAV8-fMt2 vectors at ∼8 x 10 11 or ∼4 x 10 12 viral genome-particles per mouse or AAV8-fMt2 mask viral vectors at ∼4 x 10 12 viral genome-particles per mouse. Injection of sterilized PBS vehicle was included as a control. Age and gender-matched WT littermates were included as additional controls. Mice were euthanized for analysis 3 weeks after injection. All mice were fed a standard diet containing 240-ppm iron. Each group consists of at least five mice with similar numbers of male and female. C , qRT-PCR analysis of Tmprss6 mRNA in the liver. Results are expressed as the amount relative to that of β-actin for each sample. The mean values and SD are presented. D , representative images of Western blot analysis for Neo1, the FLAG epitope of fMt2, Tfr2, and β-actin in the liver membrane preparations. E , qRT-PCR analysis of Hamp mRNA in the liver. F , serum iron assay. G ,. quantification of Neo1 bands in D . H , quantification of Tfr2 bands in D . The relative amounts to β-actin are presented. One-way ANOVA was used to analyze the data relative to WT mice. ∗∗, p < 0.01; ∗∗∗, p < 0.001; ∗∗∗∗, p < 0.0001. I , models for Neo1/Hjv induction of hepcidin expression in the absence and presence of Mt2. NEO1, neogenin.

Techniques Used: Expressing, Injection, Control, Quantitative RT-PCR, Western Blot, FLAG-tag, Membrane, Iron Assay

Increased Mt2 expression in the liver of WT mice does not reduce Neo1 . Eight-week-old 129S mice were intraperitoneally injected with AAV8-fMt2 vectors at ∼8 x 10 11 , ∼3 x 10 12 , or ∼6 x 10 12 viral genome-particles per mouse or AAV8-fMt2 mask viral vectors at ∼4 x 10 12 viral genome-particles per mouse. Injection of sterilized PBS vehicle was included as a control. Mice were euthanized for analysis 3 weeks after injection. All mice were fed a standard diet containing 240-ppm iron. Each group consists of at least five mice with similar numbers of male and female. A , qRT-PCR analysis of Tmprss6 mRNA in the liver. Results are expressed as the amount relative to that of β-actin for each sample. The mean values and SD are presented. B , representative images of Western blot analysis for Neo1, the FLAG epitope of fMt2, Tfr2, Hjv, and β-actin in the liver membrane preparations. C , qRT-PCR analysis of Hamp mRNA in the liver. D , serum iron assay. E – G , quantification of Neo1, Hjv, and Tfr2 bands in B . The relative amounts to β-actin are presented. One-way ANOVA was used to analyze the data relative to PBS-injected WT controls. ns, no statistical difference. ∗, p < 0.05; ∗∗, p < 0.01; ∗∗∗∗, p < 0.0001. NEO1, neogenin.

Figure Legend Snippet: Increased Mt2 expression in the liver of WT mice does not reduce Neo1 . Eight-week-old 129S mice were intraperitoneally injected with AAV8-fMt2 vectors at ∼8 x 10 11 , ∼3 x 10 12 , or ∼6 x 10 12 viral genome-particles per mouse or AAV8-fMt2 mask viral vectors at ∼4 x 10 12 viral genome-particles per mouse. Injection of sterilized PBS vehicle was included as a control. Mice were euthanized for analysis 3 weeks after injection. All mice were fed a standard diet containing 240-ppm iron. Each group consists of at least five mice with similar numbers of male and female. A , qRT-PCR analysis of Tmprss6 mRNA in the liver. Results are expressed as the amount relative to that of β-actin for each sample. The mean values and SD are presented. B , representative images of Western blot analysis for Neo1, the FLAG epitope of fMt2, Tfr2, Hjv, and β-actin in the liver membrane preparations. C , qRT-PCR analysis of Hamp mRNA in the liver. D , serum iron assay. E – G , quantification of Neo1, Hjv, and Tfr2 bands in B . The relative amounts to β-actin are presented. One-way ANOVA was used to analyze the data relative to PBS-injected WT controls. ns, no statistical difference. ∗, p < 0.05; ∗∗, p < 0.01; ∗∗∗∗, p < 0.0001. NEO1, neogenin.

Techniques Used: Expressing, Injection, Control, Quantitative RT-PCR, Western Blot, FLAG-tag, Membrane, Iron Assay

Ablation of both Tmprss6 and hepatic Neo1 does not affect iron induction of hepcidin expression . Five-week-old Neo1 fl/fl ; Alb-Cre + ; Tmprss6 −/− mice of both genders were fed an IDD, ICD, or HID for 4 weeks before euthanasia for analysis. These animal models were generated in the same setting of studies as described in the legend to for Neo1 fl/fl ; Alb-Cre - and Neo1 fl/fl ; Alb-Cre + mice. All presented data in this figure are generated from male mice. A , serum iron assay. One-way ANOVA was used for analysis. B , liver nonheme iron assay. Green asterisks represent one-way ANOVA analysis of the data from mice fed the same iron diet relative to the corresponding WT ( Neo1 fl/fl ; Alb-Cre - ; Tmprss6 +/+ ) controls. Red asterisks represent Two-tailed student-T test between Neo1 fl/fl ; Alb-Cre + ; Tmprss6 +/+ and Neo1 fl/fl ; Alb-Cre + ; Tmprss6 −/− mice fed the same iron diet. C , qRT-PCR analysis of Hamp mRNA levels in the liver. All qRT-PCR results are expressed as the amount relative to that of β-actin for each sample. D , serum hepcidin assay. E , qRT-PCR analysis of Id1 mRNA levels in the liver. F or G , normalized hepatic Hamp and Id1 mRNA levels to those of liver nonheme iron (C/B and E/B). Each group consists of at least four animals. The means ± SD are presented. ns, no statistical difference. ∗, p < 0.05; ∗∗, p < 0.01; ∗∗∗, p < 0.001; ∗∗∗∗, p < 0.0001. H , a model for Mt2 suppression of hepcidin expression in hepatocytes. IDD, iron deficient diet; NEO1, neogenin.

Figure Legend Snippet: Ablation of both Tmprss6 and hepatic Neo1 does not affect iron induction of hepcidin expression . Five-week-old Neo1 fl/fl ; Alb-Cre + ; Tmprss6 −/− mice of both genders were fed an IDD, ICD, or HID for 4 weeks before euthanasia for analysis. These animal models were generated in the same setting of studies as described in the legend to for Neo1 fl/fl ; Alb-Cre - and Neo1 fl/fl ; Alb-Cre + mice. All presented data in this figure are generated from male mice. A , serum iron assay. One-way ANOVA was used for analysis. B , liver nonheme iron assay. Green asterisks represent one-way ANOVA analysis of the data from mice fed the same iron diet relative to the corresponding WT ( Neo1 fl/fl ; Alb-Cre - ; Tmprss6 +/+ ) controls. Red asterisks represent Two-tailed student-T test between Neo1 fl/fl ; Alb-Cre + ; Tmprss6 +/+ and Neo1 fl/fl ; Alb-Cre + ; Tmprss6 −/− mice fed the same iron diet. C , qRT-PCR analysis of Hamp mRNA levels in the liver. All qRT-PCR results are expressed as the amount relative to that of β-actin for each sample. D , serum hepcidin assay. E , qRT-PCR analysis of Id1 mRNA levels in the liver. F or G , normalized hepatic Hamp and Id1 mRNA levels to those of liver nonheme iron (C/B and E/B). Each group consists of at least four animals. The means ± SD are presented. ns, no statistical difference. ∗, p < 0.05; ∗∗, p < 0.01; ∗∗∗, p < 0.001; ∗∗∗∗, p < 0.0001. H , a model for Mt2 suppression of hepcidin expression in hepatocytes. IDD, iron deficient diet; NEO1, neogenin.

Techniques Used: Expressing, Generated, Iron Assay, Two Tailed Test, Quantitative RT-PCR

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Journal: The Journal of Biological Chemistry

Article Title: Matriptase-2-mediated suppression of hepatic hepcidin expression in mice requires hepatocyte neogenin

doi: 10.1016/j.jbc.2026.111142

Figure Lengend Snippet: Mt2 expression markedly reduces Neo1 levels in Hep3B cells . A , diagram of Mt2 and fMt2. The MYC (m) and FLAG (f) epitopes were added to the C-terminus of Mt2. B , diagram of fNeo1. The MYC (m) and FLAG (f) epitopes were added to the C-terminus of Neo1. C , coexpression of fMt2 with fNeo1 markedly reduces fNeo1 levels in whole cell extracts (input) and on cell surface by biotinylation. AP, aprotinin. Experiments were repeated three times with consistent results. D , quantification of cell surface fNeo1 bands in C ( panel 4 ). The relative amounts to Na + K + ATPase ( panel-5 ) are presented (n = 3). The data shown are means ± SD. One-way ANOVA was used to analyze the data. ns, no statistical difference. ∗, p < 0.05; ∗∗∗∗, p < 0.0001. NEO1, neogenin.

Article Snippet: We purchased murine Neo1 ORF ( NM_008684 ) with a C-terminal FLAG/MYC epitope (fNeo1) in pCMV6 vector (#MR226235) and murine Mt2 ORF ( NM_027902.1 ) with a C-terminal FLAG/MYC epitope (fMt2) in pCMV6 vector (#MR210781) from OriGene Technologies Inc.

Techniques: Expressing

Journal: The Journal of Biological Chemistry

Article Title: Matriptase-2-mediated suppression of hepatic hepcidin expression in mice requires hepatocyte neogenin

doi: 10.1016/j.jbc.2026.111142

Figure Lengend Snippet: Hepcidin is an iron regulatory hormone that is secreted mainly by hepatocytes . A , hepcidin inhibits iron efflux from duodenum, spleen, and the liver into the circulation by blocking the plasma-membrane iron exporter, ferroportin. B , diagram of the key components that are involved in the induction of hepcidin expression in the liver. C , cartoon of NEO1 protein. It contains four immunoglobulin-like (Ig) domain, six fibronectin III domains, a transmembrane domain, and a cytoplasmic domain (CD). D , diagram of MT2 protein. Cyto: cytoplasmic domain. transmembranedomain. SEA: sea urchin sperm protein, enteropeptidase agrin. CUB: complement protein subcomponents C1r/C1s, urchin embryonic growth factor and bone morphogenetic protein one domain. L: low-density lipoprotein receptor class-A domain. Catalytic: serine protease (S/P) catalytic domain. The arrow indicates the predicted autocleavage activation site. E , iron induction of hepcidin expression by increases in Bmp6 gene transcription and TfR2 protein stabilization. F , a proposed model for the function of MT2. NEO1, neogenin.

Techniques: Blocking Assay, Clinical Proteomics, Membrane, Expressing, Activation Assay

Journal: The Journal of Biological Chemistry

Article Title: Matriptase-2-mediated suppression of hepatic hepcidin expression in mice requires hepatocyte neogenin

doi: 10.1016/j.jbc.2026.111142

Figure Lengend Snippet: Expression of exogenously administered Mt2 in the liver of Tmprss6 −/− mice increases Neo1 levels . A , diagram of Mt2, fMt2, and fMt2 mask . B , experimental design to determine the effects of exogenously administered Mt2 on Neo1 levels. Eight-week-old Tmprss6 −/− littermates on a mixed B6/129 background were intraperitoneally injected with AAV8-fMt2 vectors at ∼8 x 10 11 or ∼4 x 10 12 viral genome-particles per mouse or AAV8-fMt2 mask viral vectors at ∼4 x 10 12 viral genome-particles per mouse. Injection of sterilized PBS vehicle was included as a control. Age and gender-matched WT littermates were included as additional controls. Mice were euthanized for analysis 3 weeks after injection. All mice were fed a standard diet containing 240-ppm iron. Each group consists of at least five mice with similar numbers of male and female. C , qRT-PCR analysis of Tmprss6 mRNA in the liver. Results are expressed as the amount relative to that of β-actin for each sample. The mean values and SD are presented. D , representative images of Western blot analysis for Neo1, the FLAG epitope of fMt2, Tfr2, and β-actin in the liver membrane preparations. E , qRT-PCR analysis of Hamp mRNA in the liver. F , serum iron assay. G ,. quantification of Neo1 bands in D . H , quantification of Tfr2 bands in D . The relative amounts to β-actin are presented. One-way ANOVA was used to analyze the data relative to WT mice. ∗∗, p < 0.01; ∗∗∗, p < 0.001; ∗∗∗∗, p < 0.0001. I , models for Neo1/Hjv induction of hepcidin expression in the absence and presence of Mt2. NEO1, neogenin.

Techniques: Expressing, Injection, Control, Quantitative RT-PCR, Western Blot, FLAG-tag, Membrane, Iron Assay

Journal: The Journal of Biological Chemistry

Article Title: Matriptase-2-mediated suppression of hepatic hepcidin expression in mice requires hepatocyte neogenin

doi: 10.1016/j.jbc.2026.111142

Figure Lengend Snippet: Increased Mt2 expression in the liver of WT mice does not reduce Neo1 . Eight-week-old 129S mice were intraperitoneally injected with AAV8-fMt2 vectors at ∼8 x 10 11 , ∼3 x 10 12 , or ∼6 x 10 12 viral genome-particles per mouse or AAV8-fMt2 mask viral vectors at ∼4 x 10 12 viral genome-particles per mouse. Injection of sterilized PBS vehicle was included as a control. Mice were euthanized for analysis 3 weeks after injection. All mice were fed a standard diet containing 240-ppm iron. Each group consists of at least five mice with similar numbers of male and female. A , qRT-PCR analysis of Tmprss6 mRNA in the liver. Results are expressed as the amount relative to that of β-actin for each sample. The mean values and SD are presented. B , representative images of Western blot analysis for Neo1, the FLAG epitope of fMt2, Tfr2, Hjv, and β-actin in the liver membrane preparations. C , qRT-PCR analysis of Hamp mRNA in the liver. D , serum iron assay. E – G , quantification of Neo1, Hjv, and Tfr2 bands in B . The relative amounts to β-actin are presented. One-way ANOVA was used to analyze the data relative to PBS-injected WT controls. ns, no statistical difference. ∗, p < 0.05; ∗∗, p < 0.01; ∗∗∗∗, p < 0.0001. NEO1, neogenin.

Techniques: Expressing, Injection, Control, Quantitative RT-PCR, Western Blot, FLAG-tag, Membrane, Iron Assay

Journal: The Journal of Biological Chemistry

Article Title: Matriptase-2-mediated suppression of hepatic hepcidin expression in mice requires hepatocyte neogenin

doi: 10.1016/j.jbc.2026.111142

Figure Lengend Snippet: Ablation of both Tmprss6 and hepatic Neo1 does not affect iron induction of hepcidin expression . Five-week-old Neo1 fl/fl ; Alb-Cre + ; Tmprss6 −/− mice of both genders were fed an IDD, ICD, or HID for 4 weeks before euthanasia for analysis. These animal models were generated in the same setting of studies as described in the legend to for Neo1 fl/fl ; Alb-Cre - and Neo1 fl/fl ; Alb-Cre + mice. All presented data in this figure are generated from male mice. A , serum iron assay. One-way ANOVA was used for analysis. B , liver nonheme iron assay. Green asterisks represent one-way ANOVA analysis of the data from mice fed the same iron diet relative to the corresponding WT ( Neo1 fl/fl ; Alb-Cre - ; Tmprss6 +/+ ) controls. Red asterisks represent Two-tailed student-T test between Neo1 fl/fl ; Alb-Cre + ; Tmprss6 +/+ and Neo1 fl/fl ; Alb-Cre + ; Tmprss6 −/− mice fed the same iron diet. C , qRT-PCR analysis of Hamp mRNA levels in the liver. All qRT-PCR results are expressed as the amount relative to that of β-actin for each sample. D , serum hepcidin assay. E , qRT-PCR analysis of Id1 mRNA levels in the liver. F or G , normalized hepatic Hamp and Id1 mRNA levels to those of liver nonheme iron (C/B and E/B). Each group consists of at least four animals. The means ± SD are presented. ns, no statistical difference. ∗, p < 0.05; ∗∗, p < 0.01; ∗∗∗, p < 0.001; ∗∗∗∗, p < 0.0001. H , a model for Mt2 suppression of hepcidin expression in hepatocytes. IDD, iron deficient diet; NEO1, neogenin.

Techniques: Expressing, Generated, Iron Assay, Two Tailed Test, Quantitative RT-PCR

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